A number of different laboratory diagnostic tests called rapid influenza. Diagnostic tests (Riots) can be used for detecting the presence of influenza viruses in respiratory specimens. these includes direct viral antigen detection tests and virus isolation in cell culture but these may not be specific to detect human infection with a new influenza .a(h1n1)virus or may not yield timely results for clinical management. The recommended test for confirmation of cases is a real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) which detects influenza-specific RNA.
For confirming a case of novel influenza a (H1N1) virus infection, a sample of nasopharyngeal swab or nasal aspirate needs to be collected, using sterile synthetic tip with plastic shaft, as soon a possible after illness onset. For transporting these specimens to the laboratory, they must be taken in sterile viral transport media and placed on ice/cold packs or refrigerated. However, various factors may contribute to a lowered sensitivity for laboratory tests to detect novel influenza a (H1N1) virus in the samples. These include type of respiratory specimen (nasal 0or nasopharyngeal swab) , quantity of the specimen time from illness onset to specimen collection ,age ,of the patient ,time from specimen collection to testing ,and the storage and processing of the specimen prior to testing.
For confirming a case of novel influenza a (H1N1) virus infection, a sample of nasopharyngeal swab or nasal aspirate needs to be collected, using sterile synthetic tip with plastic shaft, as soon a possible after illness onset. For transporting these specimens to the laboratory, they must be taken in sterile viral transport media and placed on ice/cold packs or refrigerated. However, various factors may contribute to a lowered sensitivity for laboratory tests to detect novel influenza a (H1N1) virus in the samples. These include type of respiratory specimen (nasal 0or nasopharyngeal swab) , quantity of the specimen time from illness onset to specimen collection ,age ,of the patient ,time from specimen collection to testing ,and the storage and processing of the specimen prior to testing.
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